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rh bone morphogenetic protein 4 rhbmp4 r d systems 314 bp 050 rh basic fibroblast growth factor  (R&D Systems)


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    R&D Systems rh bone morphogenetic protein 4 rhbmp4 r d systems 314 bp 050 rh basic fibroblast growth factor
    Rh Bone Morphogenetic Protein 4 Rhbmp4 R D Systems 314 Bp 050 Rh Basic Fibroblast Growth Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bone+morphogenetic+factor+4/Recombinant+Human+BMP-4+Protein/pm39068648-55-200-206
    Average 97 stars, based on 1137 article reviews
    rh bone morphogenetic protein 4 rhbmp4 r d systems 314 bp 050 rh basic fibroblast growth factor - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Functional Assay:

    Article Title: Production of hepatocyte-like cells from human pluripotent stem cells.
    Article Snippet: .. Kits used for functional validations should be used as specified by the manufacturer on or after day 25 of differentiation. hPSCs (either hESCs or hIPSCs) Ca2 + - and Mg2 + -free Dulbecco’s PBS (PBS − / − ; Gibco, cat. no. 14190) Water for embryo transfer (Sigma, cat. no. W1503) Advanced DMEM/F12 (Gibco, cat. no. 12634) KnockOut serum replacement (Gibco, cat. no. 10828-028) RPMI 1640 + GlutaMAX (Gibco, cat. no. 61870) IMDM (Gibco, cat. no. 21980) F12 + GlutaMAX-1 (Gibco, cat. no. 31765) B-27 supplement containing insulin (Gibco, cat. no. 17504-044) Hepatocyte basal medium (HBM) BulletKit (Lonza, cat. no. CC-3199) MEM non-essential amino acids (MEM-NEAA; Gibco, cat. no. 1140) BSA fraction V (Europa Bioproducts, Sigma or PAA) Gelatin, porcine (Sigma-Aldrich, cat. no. G1890) β-Mercaptoethanol (Sigma-Aldrich, cat. no. M6250) FBS (Biosera, cat. no. S1818) Glutamine (Invitrogen, cat. no. 25030024) Thioglycerol ≥97% (Sigma-Aldrich, cat. no. M6145) DMSO (Sigma-Aldrich, cat. no. D2650) Poly(vinyl alcohol) (PVA; Sigma-Aldrich, cat. no. P8136) Activin A (R&D Systems, cat. no. 338-AC) Basic fibroblast growth factor (bFGF; R&D Systems, cat. no. 233-FB) Bone morphogenetic factor 4 (BMP-4; R&D Systems, cat. no. 314-BP) Oncostatin M (R&D Systems, cat. no. 295-OM) Hepatocyte growth factor (Peprotech, cat. no. 100-39) LY294002 (Promega, cat. no. V1201) CHIR99021 (StemGent, cat. no. 04-0004) Fibroblast growth factor 10 (FGF10; Autogen Bioclear, cat. no. ABC144) Transferrin (Roche, cat. no. 10652202001) Insulin (Roche, cat. no. 11376497001) Concentrated lipids, chemically defined (Gibco, cat. no. 11905) Penicillin/streptomycin (pen/strep; Gibco, cat. no. 15140122) Collagenase type IV (Invitrogen, cat. no. 17104-019) Dispase (Gibco, cat. no. 17105-041) Paraformaldehyde, 4% (wt/vol) (PFA; 16% (wt/vol) aqueous solution, methanol free; Alfa Aesar, cat. no. 43368) Donkey serum (Serotec, cat. no. CO6SB) Reagents required for the characterization of cells crItIcal Protocols for immunohistochemistry and qPCR are not given here; see refs. 5–7,10. .. AFP primary antibody (DAKO, cat. no. A0008) Albumin primary antibody (R&D Systems, cat. no. 188835) α-1-Antitrypsin primary antibody (DAKO, cat. no. A0012) CXCR4 (R&D Systems, cat. no. MAB173) SOX17 (R&D Systems, cat. no. AF1924) HNF4a (C-19) (SantaCruz, cat. no. sc6556) CK18 (cat. no. ab82254) Alexa Fluor 488 (secondary antibody); Invitrogen, cat. no. A11001 Alexa Fluor 647 (secondary antibody); Invitrogen, cat. no. A21244 Cell dissociation buffer, enzyme free, Hanks based (cat. no. 13150-016) Periodic acid–Schiff (PAS) kit (Sigma-Aldrich, cat. no. 395B-1Kit) Indocyanine green (Sigma-Aldrich, cat. no. 21980) LDL (low-density lipoprotein) kit (Cayman Chemicals, cat. no. 10011125) Albumin blue (Active Motif, cat. no. 15002) A1AT ELISA kit (Abcam, cat. no. AB108799) P450 Glo assay (Promega, cat. nos.

    Knock-Out:

    Article Title: Production of hepatocyte-like cells from human pluripotent stem cells.
    Article Snippet: .. Kits used for functional validations should be used as specified by the manufacturer on or after day 25 of differentiation. hPSCs (either hESCs or hIPSCs) Ca2 + - and Mg2 + -free Dulbecco’s PBS (PBS − / − ; Gibco, cat. no. 14190) Water for embryo transfer (Sigma, cat. no. W1503) Advanced DMEM/F12 (Gibco, cat. no. 12634) KnockOut serum replacement (Gibco, cat. no. 10828-028) RPMI 1640 + GlutaMAX (Gibco, cat. no. 61870) IMDM (Gibco, cat. no. 21980) F12 + GlutaMAX-1 (Gibco, cat. no. 31765) B-27 supplement containing insulin (Gibco, cat. no. 17504-044) Hepatocyte basal medium (HBM) BulletKit (Lonza, cat. no. CC-3199) MEM non-essential amino acids (MEM-NEAA; Gibco, cat. no. 1140) BSA fraction V (Europa Bioproducts, Sigma or PAA) Gelatin, porcine (Sigma-Aldrich, cat. no. G1890) β-Mercaptoethanol (Sigma-Aldrich, cat. no. M6250) FBS (Biosera, cat. no. S1818) Glutamine (Invitrogen, cat. no. 25030024) Thioglycerol ≥97% (Sigma-Aldrich, cat. no. M6145) DMSO (Sigma-Aldrich, cat. no. D2650) Poly(vinyl alcohol) (PVA; Sigma-Aldrich, cat. no. P8136) Activin A (R&D Systems, cat. no. 338-AC) Basic fibroblast growth factor (bFGF; R&D Systems, cat. no. 233-FB) Bone morphogenetic factor 4 (BMP-4; R&D Systems, cat. no. 314-BP) Oncostatin M (R&D Systems, cat. no. 295-OM) Hepatocyte growth factor (Peprotech, cat. no. 100-39) LY294002 (Promega, cat. no. V1201) CHIR99021 (StemGent, cat. no. 04-0004) Fibroblast growth factor 10 (FGF10; Autogen Bioclear, cat. no. ABC144) Transferrin (Roche, cat. no. 10652202001) Insulin (Roche, cat. no. 11376497001) Concentrated lipids, chemically defined (Gibco, cat. no. 11905) Penicillin/streptomycin (pen/strep; Gibco, cat. no. 15140122) Collagenase type IV (Invitrogen, cat. no. 17104-019) Dispase (Gibco, cat. no. 17105-041) Paraformaldehyde, 4% (wt/vol) (PFA; 16% (wt/vol) aqueous solution, methanol free; Alfa Aesar, cat. no. 43368) Donkey serum (Serotec, cat. no. CO6SB) Reagents required for the characterization of cells crItIcal Protocols for immunohistochemistry and qPCR are not given here; see refs. 5–7,10. .. AFP primary antibody (DAKO, cat. no. A0008) Albumin primary antibody (R&D Systems, cat. no. 188835) α-1-Antitrypsin primary antibody (DAKO, cat. no. A0012) CXCR4 (R&D Systems, cat. no. MAB173) SOX17 (R&D Systems, cat. no. AF1924) HNF4a (C-19) (SantaCruz, cat. no. sc6556) CK18 (cat. no. ab82254) Alexa Fluor 488 (secondary antibody); Invitrogen, cat. no. A11001 Alexa Fluor 647 (secondary antibody); Invitrogen, cat. no. A21244 Cell dissociation buffer, enzyme free, Hanks based (cat. no. 13150-016) Periodic acid–Schiff (PAS) kit (Sigma-Aldrich, cat. no. 395B-1Kit) Indocyanine green (Sigma-Aldrich, cat. no. 21980) LDL (low-density lipoprotein) kit (Cayman Chemicals, cat. no. 10011125) Albumin blue (Active Motif, cat. no. 15002) A1AT ELISA kit (Abcam, cat. no. AB108799) P450 Glo assay (Promega, cat. nos.

    Immunohistochemistry:

    Article Title: Production of hepatocyte-like cells from human pluripotent stem cells.
    Article Snippet: .. Kits used for functional validations should be used as specified by the manufacturer on or after day 25 of differentiation. hPSCs (either hESCs or hIPSCs) Ca2 + - and Mg2 + -free Dulbecco’s PBS (PBS − / − ; Gibco, cat. no. 14190) Water for embryo transfer (Sigma, cat. no. W1503) Advanced DMEM/F12 (Gibco, cat. no. 12634) KnockOut serum replacement (Gibco, cat. no. 10828-028) RPMI 1640 + GlutaMAX (Gibco, cat. no. 61870) IMDM (Gibco, cat. no. 21980) F12 + GlutaMAX-1 (Gibco, cat. no. 31765) B-27 supplement containing insulin (Gibco, cat. no. 17504-044) Hepatocyte basal medium (HBM) BulletKit (Lonza, cat. no. CC-3199) MEM non-essential amino acids (MEM-NEAA; Gibco, cat. no. 1140) BSA fraction V (Europa Bioproducts, Sigma or PAA) Gelatin, porcine (Sigma-Aldrich, cat. no. G1890) β-Mercaptoethanol (Sigma-Aldrich, cat. no. M6250) FBS (Biosera, cat. no. S1818) Glutamine (Invitrogen, cat. no. 25030024) Thioglycerol ≥97% (Sigma-Aldrich, cat. no. M6145) DMSO (Sigma-Aldrich, cat. no. D2650) Poly(vinyl alcohol) (PVA; Sigma-Aldrich, cat. no. P8136) Activin A (R&D Systems, cat. no. 338-AC) Basic fibroblast growth factor (bFGF; R&D Systems, cat. no. 233-FB) Bone morphogenetic factor 4 (BMP-4; R&D Systems, cat. no. 314-BP) Oncostatin M (R&D Systems, cat. no. 295-OM) Hepatocyte growth factor (Peprotech, cat. no. 100-39) LY294002 (Promega, cat. no. V1201) CHIR99021 (StemGent, cat. no. 04-0004) Fibroblast growth factor 10 (FGF10; Autogen Bioclear, cat. no. ABC144) Transferrin (Roche, cat. no. 10652202001) Insulin (Roche, cat. no. 11376497001) Concentrated lipids, chemically defined (Gibco, cat. no. 11905) Penicillin/streptomycin (pen/strep; Gibco, cat. no. 15140122) Collagenase type IV (Invitrogen, cat. no. 17104-019) Dispase (Gibco, cat. no. 17105-041) Paraformaldehyde, 4% (wt/vol) (PFA; 16% (wt/vol) aqueous solution, methanol free; Alfa Aesar, cat. no. 43368) Donkey serum (Serotec, cat. no. CO6SB) Reagents required for the characterization of cells crItIcal Protocols for immunohistochemistry and qPCR are not given here; see refs. 5–7,10. .. AFP primary antibody (DAKO, cat. no. A0008) Albumin primary antibody (R&D Systems, cat. no. 188835) α-1-Antitrypsin primary antibody (DAKO, cat. no. A0012) CXCR4 (R&D Systems, cat. no. MAB173) SOX17 (R&D Systems, cat. no. AF1924) HNF4a (C-19) (SantaCruz, cat. no. sc6556) CK18 (cat. no. ab82254) Alexa Fluor 488 (secondary antibody); Invitrogen, cat. no. A11001 Alexa Fluor 647 (secondary antibody); Invitrogen, cat. no. A21244 Cell dissociation buffer, enzyme free, Hanks based (cat. no. 13150-016) Periodic acid–Schiff (PAS) kit (Sigma-Aldrich, cat. no. 395B-1Kit) Indocyanine green (Sigma-Aldrich, cat. no. 21980) LDL (low-density lipoprotein) kit (Cayman Chemicals, cat. no. 10011125) Albumin blue (Active Motif, cat. no. 15002) A1AT ELISA kit (Abcam, cat. no. AB108799) P450 Glo assay (Promega, cat. nos.

    Real-time Polymerase Chain Reaction:

    Article Title: Production of hepatocyte-like cells from human pluripotent stem cells.
    Article Snippet: .. Kits used for functional validations should be used as specified by the manufacturer on or after day 25 of differentiation. hPSCs (either hESCs or hIPSCs) Ca2 + - and Mg2 + -free Dulbecco’s PBS (PBS − / − ; Gibco, cat. no. 14190) Water for embryo transfer (Sigma, cat. no. W1503) Advanced DMEM/F12 (Gibco, cat. no. 12634) KnockOut serum replacement (Gibco, cat. no. 10828-028) RPMI 1640 + GlutaMAX (Gibco, cat. no. 61870) IMDM (Gibco, cat. no. 21980) F12 + GlutaMAX-1 (Gibco, cat. no. 31765) B-27 supplement containing insulin (Gibco, cat. no. 17504-044) Hepatocyte basal medium (HBM) BulletKit (Lonza, cat. no. CC-3199) MEM non-essential amino acids (MEM-NEAA; Gibco, cat. no. 1140) BSA fraction V (Europa Bioproducts, Sigma or PAA) Gelatin, porcine (Sigma-Aldrich, cat. no. G1890) β-Mercaptoethanol (Sigma-Aldrich, cat. no. M6250) FBS (Biosera, cat. no. S1818) Glutamine (Invitrogen, cat. no. 25030024) Thioglycerol ≥97% (Sigma-Aldrich, cat. no. M6145) DMSO (Sigma-Aldrich, cat. no. D2650) Poly(vinyl alcohol) (PVA; Sigma-Aldrich, cat. no. P8136) Activin A (R&D Systems, cat. no. 338-AC) Basic fibroblast growth factor (bFGF; R&D Systems, cat. no. 233-FB) Bone morphogenetic factor 4 (BMP-4; R&D Systems, cat. no. 314-BP) Oncostatin M (R&D Systems, cat. no. 295-OM) Hepatocyte growth factor (Peprotech, cat. no. 100-39) LY294002 (Promega, cat. no. V1201) CHIR99021 (StemGent, cat. no. 04-0004) Fibroblast growth factor 10 (FGF10; Autogen Bioclear, cat. no. ABC144) Transferrin (Roche, cat. no. 10652202001) Insulin (Roche, cat. no. 11376497001) Concentrated lipids, chemically defined (Gibco, cat. no. 11905) Penicillin/streptomycin (pen/strep; Gibco, cat. no. 15140122) Collagenase type IV (Invitrogen, cat. no. 17104-019) Dispase (Gibco, cat. no. 17105-041) Paraformaldehyde, 4% (wt/vol) (PFA; 16% (wt/vol) aqueous solution, methanol free; Alfa Aesar, cat. no. 43368) Donkey serum (Serotec, cat. no. CO6SB) Reagents required for the characterization of cells crItIcal Protocols for immunohistochemistry and qPCR are not given here; see refs. 5–7,10. .. AFP primary antibody (DAKO, cat. no. A0008) Albumin primary antibody (R&D Systems, cat. no. 188835) α-1-Antitrypsin primary antibody (DAKO, cat. no. A0012) CXCR4 (R&D Systems, cat. no. MAB173) SOX17 (R&D Systems, cat. no. AF1924) HNF4a (C-19) (SantaCruz, cat. no. sc6556) CK18 (cat. no. ab82254) Alexa Fluor 488 (secondary antibody); Invitrogen, cat. no. A11001 Alexa Fluor 647 (secondary antibody); Invitrogen, cat. no. A21244 Cell dissociation buffer, enzyme free, Hanks based (cat. no. 13150-016) Periodic acid–Schiff (PAS) kit (Sigma-Aldrich, cat. no. 395B-1Kit) Indocyanine green (Sigma-Aldrich, cat. no. 21980) LDL (low-density lipoprotein) kit (Cayman Chemicals, cat. no. 10011125) Albumin blue (Active Motif, cat. no. 15002) A1AT ELISA kit (Abcam, cat. no. AB108799) P450 Glo assay (Promega, cat. nos.

    Recombinant:

    Article Title: Derivation of neural crest cells from human pluripotent stem cells.
    Article Snippet: 688 | VOL.5 NO.4 | 2010 | nature protocols IntroDuctIon Human embryonic stem cells and neural crest differentiation Since the first successful derivation of human embryonic stem cells (hESCs) by Thomson and colleagues, hESCs have become a valuable in vitro model of early human development. hESCs are characterized by their ability to differentiate into cell types of all three germ layers.. Neural differentiation of hESCs is associated with the formation of neural rosettes, columnar epithelial cells representing the early developing neuroepithelium. hESC-derived neuroepithelial cells are regarded as an in vitro source of neural (stem) cells capable of generating the various neural cell types that comprise the central nervous system (CNS).. In addition to their CNS differentiation potential, neural rosettes can also give rise to cells of neural crest (NC) identity.



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    Overview of the hepatic differentiation timeline, the final concentration of growth factors and small molecules used

    Journal: STAR Protocols

    Article Title: Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays

    doi: 10.1016/j.xpro.2021.100471

    Figure Lengend Snippet: Overview of the hepatic differentiation timeline, the final concentration of growth factors and small molecules used

    Article Snippet: Bone morphogenetic factor 4 (BMP4) , Miltenyi Biotec , Cat # 130-111-168.

    Techniques: Concentration Assay

    Journal: STAR Protocols

    Article Title: Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays

    doi: 10.1016/j.xpro.2021.100471

    Figure Lengend Snippet:

    Article Snippet: Bone morphogenetic factor 4 (BMP4) , Miltenyi Biotec , Cat # 130-111-168.

    Techniques: Recombinant, Saline, Cell Culture, Knock-Out, Passaging, Transfection, Sterility, Transferring